Composite

Part:BBa_K3484001:Design

Designed by: Eduard Sune & Andreu Pascuet   Group: iGEM20_UPF_Barcelona   (2020-10-26)


Intein-mediated T3(THRB) → sfGFP (transcriptional unit)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 235
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 722
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 278
    Illegal SapI.rc site found at 1637


Design Notes

Special considerations have been taken into account for the construction of the protein domain and can be seen at the registry of the basic part BBa_K3484000.

Source

Parts used for this composite are all from previous entries of the iGEM Registry:

References

Look for references at BBa_K3484000.